LIMK1(Phospho-Thr508) Antibody

Catalog Number:

11126

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P53667
NCBI Protein: NP_002305.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.

Applications:

WB IHC IF

Background:

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

References:

appl_detail:

Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF