Product Datasheet  
LIMK1(Phospho-Thr508) Antibody  
Catalog Number: 11126  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P53667
    NCBI Protein: NP_002305.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 72kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • LIMK-1; kinase LIMK1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
  • Applications：  
  • WB IHC IF
  • Background：  

  • Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

    Soosairajah J, et al. (2005) EMBO J.
    Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
    Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.




 
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