Akt2(Phospho-Ser474) Antibody

Catalog Number:

11124

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P31751
NCBI Protein: NP _001617.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.

Applications:

WB IHC

Background:

General protein kinase capable of phosphorylating several known proteins.

Sun M, et al. (2001) Cancer Res; 61(16): 5985-91.
Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30.
Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.

References:

appl_detail:

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF