Product Datasheet  
Akt2(Phospho-Ser474) Antibody  
Catalog Number: 11124  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P31751
    NCBI Protein: NP _001617.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • PKB beta; Protein kinase B; RAC-PK-beta
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
  • Applications：  
  • WB IHC
  • Background：  

  • General protein kinase capable of phosphorylating several known proteins.

    Sun M, et al. (2001) Cancer Res; 61(16): 5985-91.
    Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30.
    Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.




 
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