Myosin Light Chain 2 (Phospho-Ser19) Antibody

Catalog Number:

11114

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P19105
NCBI Protein: NP_006088.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 19.

Applications:

WB IHC

Background:

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

References:

appl_detail:

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF