Product Datasheet  
Myosin Light Chain 2 (Phospho-Ser19) Antibody  
Catalog Number: 11114  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P19105
    NCBI Protein: NP_006088.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 2.
  • reactivity：  
  • Hu Rt
  • appl_detail：  

  • Predicted MW: 18kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • MLC-2a; MLC2a; MLRA; MYL7; MYLC2A
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 19.
  • Applications：  
  • WB IHC
  • Background：  

  • Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

    Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
    Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
    Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256




 
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