p53(Phospho-Ser15) Antibody

Catalog Number:

11094

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P04637
NCBI Protein: NP_000537.3

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 15 (P-L-S(p)-Q-E) derived from Human p53.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of p53 only whenphosphorylated at serine15.

Applications:

WB IHC IF

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

References:

appl_detail:

Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF