Product Datasheet  
p53(Phospho-Ser15) Antibody  
Catalog Number: 11094  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P04637
    NCBI Protein: NP_000537.3
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 15 (P-L-S(p)-Q-E) derived from Human p53.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Predicted MW: 53kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p53 only whenphosphorylated at serine15.
  • Applications：  
  • WB IHC IF
  • Background：  

  • Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

    Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
    Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
    Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
    Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.




 
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