Paxillin(Phospho-Tyr118) Antibody

Catalog Number:

11089

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P49023
NCBI Protein: NP_001074324.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.

Applications:

WB IHC

Background:

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Tao WA, et al.(2005)Nat Methods.2(8): 591-598.
Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250.
Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522.
Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.

References:

appl_detail:

Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF