Product Datasheet  
Paxillin(Phospho-Tyr118) Antibody  
Catalog Number: 11089  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P49023
    NCBI Protein: NP_001074324.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 68kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • PAXI; PXN;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.
  • Applications：  
  • WB IHC
  • Background：  
  • Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

    Tao WA, et al.(2005)Nat Methods.2(8): 591-598.
    Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250.
    Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522.
    Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.




 
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