6-His Antibody

Catalog Number:

35533

Amount:

100μg

Price:

$319

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide: 18XHis conjugated to KLH

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects transfected proteins contanining 6-His tag.

Applications:

WB

Background:

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

References:

appl_detail:

Western blotting: 1:500-1:2000

Datasheet PDF