Product Datasheet  
6-His Antibody  
Catalog Number: 35533  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Form of Antibody：  
  • Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthetic peptide: 18XHis conjugated to KLH
  • reactivity：  
  • appl_detail：  
  • Western blotting: 1:500-1:2000
  • other_names：  
  • 6 His epitope tag
  • Purification：  
  • Antigen affinity purification.
  • Specificity：  
  • The antibody detects transfected proteins contanining 6-His tag.
  • Applications：  
  • WB
  • Background：  
  • The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.



 
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