HER2(Phospho-Tyr877) Antibody

Catalog Number:

15175

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P04626
NCBI Protein: NP_001005862.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.

Applications:

WB IHC IF

Background:

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.

References:

appl_detail:

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF