Product Datasheet  
HER2(Phospho-Tyr877) Antibody  
Catalog Number: 15175  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P04626
    NCBI Protein: NP_001005862.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 185kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • C-erbB-2; ErbB2;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

    Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
    Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
    Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.



 
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