Ezrin(Phospho-Tyr353) Antibody

Catalog Number:

11063

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15311
NCBI Protein: NP_001104547.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Applications:

WB IHC

Background:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305.
Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
 

References:

appl_detail:

Predicted MW: 81kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Datasheet PDF