Product Datasheet  
Ezrin(Phospho-Tyr353) Antibody  
Catalog Number: 11063  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P15311
    NCBI Protein: NP_001104547.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Predicted MW: 81kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
     

  • other_names：  
  • Cytovillin; EZRI; VIL2; Villin 2; p81
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.
  • Applications：  
  • WB IHC
  • Background：  

  • Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

    Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490.
    Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743.
    Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305.
    Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
     




 
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