RIP Antibody

Catalog Number:

35482

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q13546;
NCBI Gene#:8737

Form of Antibody:

Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant fragment corresponding to a region within amino acids 371 and 635 of RIP.

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RIP protein.

Applications:

WB

Background:

Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

References:

appl_detail:

Western blotting: 1:500-1:3000

Datasheet PDF