Pyruvate Kinase (liver/RBC) Antibody

Catalog Number:

35442

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P30613;
NCBI Gene#:5313

Form of Antibody:

Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant fragment corresponding to a region within amino acids 1 and 301 of Pyruvate Kinase .

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Pyruvate Kinase (liver/RBC) protein.

Applications:

WB

Background:

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

References:

appl_detail:

Western blotting: 1:500-1:20000

Datasheet PDF