Grp78 Antibody

Catalog Number:

35394

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P11021;
NCBI Gene#:3309

Form of Antibody:

Rabbit IgG in 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant fragment corresponding to a region within amino acids 478 and 654 of Grp78.

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Grp78 protein.

Applications:

WB

Background:

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

References:

appl_detail:

Western blotting: 1:500-1:3000

Datasheet PDF