MDM2 Antibody

Catalog Number:

35346

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q00987;
NCBI Gene#:4193

Form of Antibody:

Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Synthetic peptide contain a sequence corresponding to a region within amino acids 98 and 161 of MDM2.

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MDM2 protein.

Applications:

WB

Background:

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]

References:

appl_detail:

Western blotting: 1:500-1:3000

Datasheet PDF