Rad17 Antibody

Catalog Number:

35336

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:O75943;
NCBI Gene#:5884

Form of Antibody:

Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant fragment corresponding to a region within amino acids 389 and 681 of Rad17.

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Rad17 protein.

Applications:

WB

Background:

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq]

References:

appl_detail:

Western blotting: 1:500-1:3000

Datasheet PDF