IRF-3 (Ab-396) Antibody

Catalog Number:

21681

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:Q14653
Gene ID:3661

Form of Antibody:

Storage/Stability:

Immunogen:

Peptide sequence around aa.394-398(H-I-S-N-S) derived from Human IRF-3.

Purification:

Specificity/Sensitivity:

The antibody detects endogenous level of total IRF-3 protein.

Applications:

WB

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

References:

appl_detail:

Predicted MW: 45-55kd
Western blotting: 1: 500~1: 1000

Datasheet PDF