Product Datasheet  
IRF-3 (Ab-396) Antibody  
Catalog Number: 21681  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot:Q14653
    Gene ID:3661
  • Form of Antibody：  
  • Storage：  
  • Immunogen：  
  • Peptide sequence around aa.394-398(H-I-S-N-S) derived from Human IRF-3.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 45-55kd
    Western blotting: 1: 500~1: 1000
  • other_names：  
  • interferon regulatory factor 3,
  • Purification：  
  • Specificity：  
  • The antibody detects endogenous level of total IRF-3 protein.
  • Applications：  
  • WB
  • Background：  
  • Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.



 
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