REC8 Antibody

Catalog Number:

34779

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O95072
NCBI Gene ID: 9985

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human REC8.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total REC8 protein.

Applications:

WB

Background:

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II By similarity.

Parisi S., Mol. Cell. Biol. 19:3515-3528(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF