MGMT Antibody

Catalog Number:

34286

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P16455
NCBI Gene ID: 4255

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human MGMT.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MGMT protein.

Applications:

WB

Background:

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Tano K., Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B., J. Biol. Chem. 265:9563-9569(1990).
Koike G., J. Biol. Chem. 265:14754-14762(1990)

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF