Product Datasheet  
MGMT Antibody  
Catalog Number: 34286  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P16455
    NCBI Gene ID: 4255
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from internal of human MGMT.
  • reactivity：  
  • Hu Rt
  • appl_detail：  
  • Western blotting: 1:500~1:3000

  • other_names：  
  • Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT;
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total MGMT protein.
  • Applications：  
  • WB
  • Background：  
  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

    Tano K., Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
    Rydberg B., J. Biol. Chem. 265:9563-9569(1990).
    Koike G., J. Biol. Chem. 265:14754-14762(1990)



 
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