PARD3 Antibody

Catalog Number:

33992

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q8TEW0
NCBI Gene ID: 56288

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human PARD3.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PARD3 protein.

Applications:

WB IF

Background:

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

Joberty G., Nat. Cell Biol. 2:531-539(2000).
Fang C.M., Cell Res. 11:223-229(2001).
Kohjima M., Biochem. Biophys. Res. Commun. 299:641-646(2002).

References:

appl_detail:

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Datasheet PDF