XRCC4 Antibody

Catalog Number:

33549

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13426
NCBI Gene ID: 7518

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human XRCC4.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total XRCC4 protein.

Applications:

WB

Background:

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Ling Chen, J. Biol. Chem., Aug 2000; 275: 26196.
J¨¦r?me Drouet, J. Biol. Chem., Feb 2005; 280: 7060 - 7069.
Ulf Grawunder, J. Biol. Chem., Sep 1998; 273: 24708.
Ray Leber, J. Biol. Chem., Jan 1998; 273: 1794.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF