Uba2 Antibody

Catalog Number:

33535

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9UBT2
NCBI Gene ID: 10054

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from C-terminal of human Uba2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Uba2 protein.

Applications:

WB IHC

Background:

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Mark H. L. Lambermon, Mol. Cell. Biol., Jun 2002; 22: 4346 - 4357.
Kyoung-Seok Ryu, J. Biol. Chem., Sep 2003; 278: 36621 - 36627.
R. Jrgen Dohmen, J. Biol. Chem., Jul 1995; 270: 18099.
Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF