Topoisomerase II β Antibody

Catalog Number:

33528

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q02880
NCBI Gene ID: 7155

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human topoisomerase II β.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total topoisomerase II β protein.

Applications:

WB IHC

Background:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Ren¨¦ M. Linka, Nucleic Acids Res., May 2007; 10.1093.
John R. Jenkins, Nucleic Acids Res., Nov 1992; 20: 5587 - 5592.
Kathryn L. Gilroy, Nucleic Acids Res., Mar 2006; 34: 1597 - 1607.
Sophie Dereuddre, Cancer Res., Oct 1997; 57: 4301 - 4308.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF