PIAS4 Antibody

Catalog Number:

33518

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q8N2W9
NCBI Gene ID: 51588

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human PIAS4.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PIAS4 protein.

Applications:

WB IHC

Background:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.

Zendra E. Zehner, AACR Meeting Abstracts, Apr 2005; 2005: 456.
Amador Albor, J. Biol. Chem., Sep 2006; 281: 25850 - 25866.
Christina-Maria Hecker, J. Biol. Chem., Jun 2006; 281: 16117 - 16127.
Yun Zhao, Stem Cells, Apr 2007; 10.1634.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF