MCM2 Antibody

Catalog Number:

33431

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P49736
NCBI Gene ID: 4171

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human MCM2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MCM2 protein.

Applications:

WB IHC

Background:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

H Yan, Genes & Dev., Jun 1991; 5: 944 - 957.
Yukio Ishimi, J. Biol. Chem., Nov 2001; 276: 42744 - 42752.
Muluye E. Liku, Mol. Biol. Cell, Oct 2005; 16: 5026 - 5039.
Daniela S. Dimitrova, J. Cell Biol., Aug 1999; 146: 709.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF