Product Datasheet  
MCM2 Antibody  
Catalog Number: 33431  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P49736
    NCBI Gene ID: 4171
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from human MCM2.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • BM28; CDCL1; DNA replication licensing factor MCM2; KIAA0030; MCMD2
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total MCM2 protein.
  • Applications：  
  • WB IHC
  • Background：  
  • Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

    H Yan, Genes & Dev., Jun 1991; 5: 944 - 957.
    Yukio Ishimi, J. Biol. Chem., Nov 2001; 276: 42744 - 42752.
    Muluye E. Liku, Mol. Biol. Cell, Oct 2005; 16: 5026 - 5039.
    Daniela S. Dimitrova, J. Cell Biol., Aug 1999; 146: 709.



 
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