E2F4 Antibody

Catalog Number:

33361

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q16254
NCBI Gene ID: 1874/1875

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human E2F4.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total E2F4 protein.

Applications:

WB IHC

Background:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1.

Kathryn M. Kinross, Blood, Aug 2006; 108: 886 - 895.
Dawei Wang, Mol. Cell. Biol., May 2000; 20: 3417 - 3424.
Stephan P. Persengiev, Mol. Cell. Biol., Sep 1999; 19: 6048 - 6056.
Stefan Gaubatz, Mol. Cell. Biol., Feb 2001; 21: 1384 - 1392.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF