DNA Polymerase β Antibody

Catalog Number:

33357

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P06746
NCBI Gene ID: 5423

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human DNA Polymerase β.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total DNA Polymerase β protein.

Applications:

WB IHC

Background:

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Hiroyuki Matsumoto, Plant Cell Physiol., Nov 1981; 22: 1317 - 1324.
L.K. Karagyozov, Nucleic Acids Res., Jun 1978; 5: 1907 - 1917.
F Costanzo, J. Virol., Mar 1977; 21: 996 - 1001.
AJ Faber, Eur. J. Biochem., Nov 1981; 120: 357.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF