Collagen IV Antibody

Catalog Number:

33342

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P02462
NCBI Gene ID: 1282

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human Collagen IV.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Collagen IV protein.

Applications:

WB IHC IF

Background:

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

Mary K. Chelberg, Cancer Res., Sep 1989; 49: 4796 - 4802.
Patricia L. Graham, J. Cell Biol., Jun 1997; 137: 1171.
PJ Lein, J. Cell Biol., Apr 1991; 113: 417.
EC Tsilibary, J. Biol. Chem., Dec 1988; 263: 19112 - 19118.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

IF 1:100-1:500

Datasheet PDF