Product Datasheet  
Collagen IV Antibody  
Catalog Number: 33342  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P02462
    NCBI Gene ID: 1282
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from human Collagen IV.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100

    IF 1:100-1:500

  • other_names：  
  • COL4A1 NC1 domain; COLLAGEN OF BASEMENT MEMBRANE; ALPHA-1 CHAIN ARRESTEN; alpha 1 type IV collagen preproprotein; collagen IV alpha-1 polypeptide
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total Collagen IV protein.
  • Applications：  
  • WB IHC IF
  • Background：  

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

    Mary K. Chelberg, Cancer Res., Sep 1989; 49: 4796 - 4802.
    Patricia L. Graham, J. Cell Biol., Jun 1997; 137: 1171.
    PJ Lein, J. Cell Biol., Apr 1991; 113: 417.
    EC Tsilibary, J. Biol. Chem., Dec 1988; 263: 19112 - 19118.




 
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