Bax Antibody

Catalog Number:

33327

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q07812
NCBI Gene ID: 581/581

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human Bax.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Bax protein.

Applications:

WB IHC IF

Background:

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Francesca Andriani, J Natl Cancer Inst, Sep 2001; 93: 1314 - 1324.
H Martinez-Valdez, J. Exp. Med., Mar 1996; 183: 971.
Joslyn K. Brunelle, J. Biol. Chem., Feb 2004; 279: 4305 - 4312.
Renren Wen, Mol. Cell. Biol., Jan 2001; 21: 678 - 689.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF