APAF-1-ALT Antibody

Catalog Number:

33324

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O14727-6
NCBI Gene ID: 317

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human APAF-1-ALT.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total APAF-1-ALT protein.

Applications:

WB IHC IF

Background:

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140 - 12146.
Liam O¡¯Connor, Cancer Res., Mar 2000; 60: 1217 - 1220.
Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248 - 2258
Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824 - 10832.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF