Product Datasheet  
APAF-1-ALT Antibody  
Catalog Number: 33324  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O14727-6
    NCBI Gene ID: 317
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from human APAF-1-ALT.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total APAF-1-ALT protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

    Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140 - 12146.
    Liam O¡¯Connor, Cancer Res., Mar 2000; 60: 1217 - 1220.
    Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248 - 2258
    Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824 - 10832.



 
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