B-Raf (Ab-753) Antibody

Catalog Number:

33277

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15056
NCBI Gene ID: 673

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human B-Raf around the phosphorylation site of threonine 735 (P-K-T(p)-P-I).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total B-Raf protein.

Applications:

WB

Background:

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Hillier L.W., Nature 424:157-164(2003).
Eychene A., Oncogene 7:1657-1660(1992).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF