APLF (Ab-116) Antibody

Catalog Number:

33273

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q8IW19
NCBI Gene ID: 200558

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total APLF protein.

Applications:

WB IHC

Background:

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF