Product Datasheet  
APLF (Ab-116) Antibody  
Catalog Number: 33273  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q8IW19
    NCBI Gene ID: 200558
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • APFL; aprataxin and PNK-like factor; C2orf13; Xip1;
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total APLF protein.
  • Applications：  
  • WB IHC
  • Background：  
  • Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

    Saunders W.S., J. Cell Sci. 104:573-582(1993).
    Ye Q., J. Biol. Chem. 271:14653-14656(1996).
    Ye Q., J. Biol. Chem. 272:14983-14989(1997).



 
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