AML1 (Ab-435) Antibody

Catalog Number:

33271

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q01196
NCBI Gene ID: 861

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total AML1 protein.

Applications:

WB

Background:

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
Nucifora G., Blood 81:2728-2734(1993).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF