Product Datasheet  
AML1 (Ab-435) Antibody  
Catalog Number: 33271  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q01196
    NCBI Gene ID: 861
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized non-phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T).
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500~1:3000

  • other_names：  
  • Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total AML1 protein.
  • Applications：  
  • WB
  • Background：  
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

    Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
    Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
    Nucifora G., Blood 81:2728-2734(1993).



 
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