AurB/C (Ab-202/175) Antibody

Catalog Number:

33177

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9UQB9
NCBI Gene ID: 9212/6795

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human AurB/C around the phosphorylation site of threonine 202/175 (C-G-T(p)-L-D).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total AurB/C protein.

Applications:

WB

Background:

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.

Tseng T.-C., DNA Cell Biol. 17:823-833(1998).
Bernard M., Genomics 53:406-409(1998).
Kimura M., J. Biol. Chem. 274:7334-7340(1999)

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF