MITF (Ab-180/73) Antibody

Catalog Number:

33138

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O75030
NCBI Gene ID: 4286

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MITF protein.

Applications:

WB IHC IF

Background:

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

J¨®n H. Hallsson, Genetics, May 2004; 167: 233 - 241.
Eiichi Morii, Blood, Apr 2001; 97: 2038 - 2044.
Eiichi Morii, Blood, Sep 2004; 104: 1656 - 1661.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF