Product Datasheet  
MITF (Ab-180/73) Antibody  
Catalog Number: 33138  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O75030
    NCBI Gene ID: 4286
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Western blotting: 1:500~1:3000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:500
  • other_names：  
  • MITF M1; Microphthalmia-associated transcription factor;
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total MITF protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

    J¨®n H. Hallsson, Genetics, May 2004; 167: 233 - 241.
    Eiichi Morii, Blood, Apr 2001; 97: 2038 - 2044.
    Eiichi Morii, Blood, Sep 2004; 104: 1656 - 1661.



 
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