Product Datasheet  
syk(Ab-323) Antibody  
Catalog Number: 21546  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P43405
    NCBI Protein: NP_001128524.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 72kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • Spleen tyrosine kinase
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total syk protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

    Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
    Turner, M. et al. (2000) Immunol. Today 21, 148-154.
    Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
    Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.



 
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