Product Datasheet  
Cyclin B1(Ab-147) Antibody  
Catalog Number: 21540  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P14635
    NCBI Protein: NP_114172.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total Cyclin B1 protein.
  • Applications：  
  • WB IHC IF
  • Background：  

  • The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

    Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
    Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
    Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.




 
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